cypX [2017-8-11 16:0:3]

cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase

locus: BSU35060

pI: 5.31

mw: 45.31 kDa

protein length: 405 aa

gene length: 1215 bp

function: biosynthesis of the extracellular iron chelate pulcherrimin

product: cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]

Gene

Coordinates: 3,602,588 → 3,603,805

The protein

Catalyzed reaction/ biological activity

• implicated in the three-step oxidative transformation of the diketopiperazine cyclo-l-leucyl-l-leucyl into pulcherriminic acid [Pubmed|20690619]

Protein family

• [SW|cytochrome P450] family (according to Swiss-Prot)

Structure

• [PDB|3NC3] [Pubmed|20690619]

Expression and Regulation

Operons

• genes: [gene|BCE1D8CDDF07396583FF50C4C192785545244BE2|yvmC]-[gene|D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53|cypX]
  description: [http://www.liebertonline.com/doi/pdf/10.1089/ind.2006.2.66 Reference]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|0D555F2AB7DC863E6FF388888308E980514DB719|PchR]: repression, in [regulon|0D555F2AB7DC863E6FF388888308E980514DB719|PchR regulon]

  regulation

  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• MGNA-A333 (cypX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/333 NBRP B. subtilis, Japan]

References

• 20690619,20817675,4204912,27542896